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B6.129S7-Rag1tm1Mom/J
品系货号：002216 ｜ 通用名称：Rag1 KO 小鼠
此品系为 Rag1tm1Mom 突变纯合小鼠，不会产生成熟 T 细胞或成熟 B 细胞。 其表型可描述为“无渗漏”免疫缺陷。需要注意的是，C57BL/6J 遗传背景的 Rag1 基因敲除品系也可通过品系货号 034159 获得。
品系特点
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同源的
靶向突变
捐赠者 IMR Colony - 杰克森实验室
原始参考文献
如果在研究中使用了Rag1敲除小鼠品系，请在文章发表时引用原始文献，并在材料与方法部分提及JAX品系号002216。
查阅参考文献 > 如果在研究中使用了Rag1敲除小鼠品系，请在文章发表时引用原始文献，并在材料与方法部分提及JAX品系号002216。品系详情
此品系为 Rag1tm1Mom 突变纯合小鼠，不会产生成熟 T 细胞或成熟 B 细胞。Prkdcscid 纯合小鼠可以产生一些 B 细胞和 IgM（即，“渗漏”），Rag1tm1Mom 纯合小鼠则缺乏所有类型的成熟淋巴细胞（即，“无渗漏”）。Rag1 敲除小鼠没有 CD3+ 或 T 细胞受体 (T cell receptor, TCR) αβ阳性细胞。杂合子或野生型同窝小鼠胸腺中细胞含量是突变小鼠的 15 到 130 倍。胸腺细胞为 CD8阴性CD4阴性 且大多为 IL2受体阳性。脾脏及骨髓中均不含 IgM 或 IgD 染色阳性的细胞，指示成熟 B 细胞完全缺失。这些数据和其他数据表明，B 细胞和 T 细胞发育在早期已经被阻断。通过肉眼观察难以区分该突变体与杂合子或正常野生型同胞小鼠。
品系建立
Rag1tm1Mom 突变品系由麻省理工学院癌症研究中心 Susumu Tonegawa 博士实验室的 Peter Mombaerts 博士建立。在品系建立过程中，使用了带 Pgk-neo 标记的靶向载体。该靶向载体引起的同源重组导致编码序列 5' 端有 1356 bp 长度的缺失。使用的是源自 129S7/SvEvBrd-Hprt+ 的 AB1 胚胎干细胞系。 将携带 Rag1tm1Mom 突变的小鼠与 C57BL/6J 近交小鼠回交十代，即获得背景为C57BL/6J的品系。
参考文献
精选参考文献
如果在研究中使用了Rag1敲除小鼠品系，请在文章发表时引用原始文献，并在材料与方法部分提及JAX品系号002216。
1992
RAG-1-deficient mice have no mature B and T lymphocytes.
Mombaerts P , et al.
健康报告 屏障设施级别描述 >
育种须知 更多繁育和饲养支持 >
Rag1tm1Mom品系通过纯合子配繁建立种群。该品系需要饲养在与Prkdcscid纯合小鼠或其它免疫缺陷品系相同的无特定病原体（SPF）环境下。仅能订购纯合小鼠。
繁育策略
纯合子X纯合子
销售和使用条款
002216
Rag1 KO
肿瘤免疫，癌症研究，免疫相关研究
参考文献|
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精选参考文献如果在研究中使用了Rag1敲除小鼠品系，请在文章发表时引用原始文献，并在材料与方法部分提及JAX品系号002216。
1992RAG-1-deficient mice have no mature B and T lymphocytes.
Mombaerts P , et al. -
其它参考文献
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