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DBA/2J
品系货号：000671｜ 通用名称：DBA2
又名： D2J, D2, DBA, DBA/2
DBA/2J小鼠是一种广泛使用的近交品系。 此品系具有以下特征：不易发生动脉粥样硬化病变、高频听力丧失、易发生听源性惊厥、进展性眼部异常（与人类遗传性青光眼相似）、对甲基苯丙胺有亲和能力以及对酒精和吗啡极度不耐受。
重点提示：本品系为Cdh23ahl纯合子，即携带年龄相关的听力丧失1纯合子突变。在该品系上的这种纯合子突变会导致在3月龄时已出现的重度听力损失会进一步发展。
品系特点 品系详情 品系建立 参考文献 基因分型和繁育 销售和使用条款
 
 
品系特点
近交系
受遗传稳定性项目保护
  
附加使用限制
原始参考文献
Info 在文章发表时，如使用了该品系，请引用原始文献，并在材料与方法部分注明JAX品系号000671。
查阅参考文献 >
  
品系详情
DBA/2J小鼠是一种广泛使用并极具研究价值的近交品系，其应用领域包括心血管生物学、神经生物学和感觉神经研究。 此品系的特点通常与C57BL/6J近交品系（品系货号：000664）形成对比。 DBA/2J小鼠在接受致动脉粥样硬化饮食（1.25%胆固醇、0.5%胆酸和15%脂肪）14周后，表现出对动脉粥样硬化病变的低易感性（20到350um2动脉粥样硬化病变/主动脉横截面）。此外，DBA/2J 小鼠大约在离乳/青春期（3-4周龄）开始出现高频听力丧失，并在2-3月龄时发展为重度听力丧失。 在1951-1975年期间，DBA/2J小鼠品系出现了自发的年龄相关性听力丧失8突变。 该品系具有三种可引起进展性耳蜗病状的隐性等位基因，最初影响柯蒂氏器。 随着前腹侧耳蜗核体积减小和神经元损失，周围性听力丧失出现同步进展。 年轻DBA/2J近交系小鼠携带asp2突变，因此也容易发生听源性惊厥，然而这种易感性会随着小鼠成年而降低。许多DBA/2J小鼠都会发展为钙化性心包炎，睾丸、舌头和骨骼肌均可见钙化病变。 此品系对植物血凝素反应最弱 (Heiniger et al, 1975)，但对氟哌啶醇高度敏感 (kane et al, 1993)。
DBA/2J小鼠随着衰老逐渐出现进展性眼部异常，与人类遗传性青光眼非常相似。 眼部异常包括虹膜色素分散、虹膜萎缩、虹膜前粘连（虹膜与角膜粘连）和眼压升高 (IOP)。 DBA/2J小鼠从3-4个月龄开始出现疾病症状，56%的雌鼠和15%的雄鼠在此时出现虹膜色素上皮细胞丢失和周边虹膜透光的表现。 到6-7月龄时，所有小鼠都表现出明显的广泛透光和虹膜边界增厚。 部分雌鼠在6月龄时眼压明显升高。 到9月龄时，雌雄小鼠均表现出眼压升高，其中雌鼠（平均值： 20.3 +/- 1.8 mmHg）高于雄鼠（平均值： 16.2 +/- 1.4 mmHg）。 视网膜组织病理学显示视网膜神经节细胞、γ-氨基丁酸 (GABA) 能神经元和胆碱能无长突细胞丢失 (Moon et al, 2005)。 GpnmbR150X和Tyrp1isa等位基因都存在于DBA/2J小鼠中，共同促进了小鼠的眼睛表型。
此外，DBA/2J小鼠由于自发的Taar1m1J点突变表现出甲基苯丙胺亲和性，但对酒精和吗啡极度不耐受 (Belknap et al, 1993)。 2002年，Vance等报道了DBA/2J小鼠NK细胞的特点，即缺乏CD94/NKG2A受体的表面表达。CD94/NKG2受体通常表达于大多数胎儿NK细胞的表面。 认为CD94/NKG2的表达与NK细胞自身耐受和区分MHC I类分子低/高表达靶细胞的能力有关。CD94是小鼠Klrd1位点的产物，该基因位于小鼠6号染色体。 Wilhelm及其同事随后发表了一篇文章 (Wilhelm et al, 2003)，在其中报道了DBA/2J小鼠Klrd1基因3'末端的缺失。 这个约2.4 kb的缺失并不影响基因的转录，但会阻碍CD94蛋白的翻译和细胞表面表达。 对杰克森实验室保存的DNA样本的分析（结果未发表）证实了DBA/2J品系中存在Klrd1缺失。 该缺失发生于1984年至1989年之间，在我们的鼠群中为纯合子，这使得DBA/2J 小鼠天生缺乏 CD94。
品系建立
DBA近交品系是最古老的近交小鼠品系。1909 年，CC Little博士从一个根据被毛颜色分离的鼠群开始近亲交配。 在1929年和1930年进行亚系杂交，建立了包含DBA/1和 DBA/2在内的多个新亚系。DBA/1和DBA/2在多个位点上存在差异（包括MHC H2单倍型），这很可能是分离亚系时品系中残留杂合性的结果。
参考文献
精选参考文献
在文章发表时，如使用了该品系，请引用原始文献，并在材料与方法部分注明JAX品系号000671。
2017

A Spontaneous Mutation in Taar1 Impacts Methamphetamine-Related Traits Exclusively in DBA/2 Mice from a Single Vendor.

Reed C , et al.

PubMed:29403379

MGI：J：84847

Front Pharmacol 8:993

2017

Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains.

Timmermans S , et al.

PubMed:28784771

MGI:J:244295

Proc Natl Acad Sci U S A 114(34):9158-9163

2013

Deficiency of complement component 5 ameliorates glaucoma in DBA/2J mice.

Howell GR , et al.

PubMed:23806181

MGI:J:199369

J Neuroinflammation 10(1):76

 
2013

Retinal ganglion cell dendritic atrophy in DBA/2J glaucoma.

Williams PA , et al.

PubMed:23977271

MGI:J:199858

PLoS One 8(8):e72282

 
2013

Deficiency of complement component 5 ameliorates glaucoma in DBA/2J mice.

Howell GR , et al.

PubMed:23806181

MGI:J:199369

J Neuroinflammation 10(1):76

 
2008

Mouse genetic models: an ideal system for understanding glaucomatous neurodegeneration and neuroprotection.

Howell GR , et al.

PubMed:18929118

MGI:J:140472

Prog Brain Res 173:303-21

 
2004

Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse.

Petkov PM , et al.

PubMed:15081119

MGI:J:89298

Genomics 83(5):902-11

 
2002

Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice.

Anderson MG , et al.

PubMed:11743578

MGI:J:75398

Nat Genet 30(1):81-5

 
2001

Genetic loci determining bone density in mice with diet-induced atherosclerosis.

Drake TA , et al.

PubMed:11328966

MGI:J:69682

Physiol Genomics 5(4):205-15

 
1995

Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes.

Kirk EA , et al.

PubMed:7595076

MGI:J:28648

J Lipid Res 36(7):1522-32

 
浏览所有参考文献 >
基因分型和繁育
 
基因分型 基因分型相关资源>
Allele Symbol Taar1 rs33645709-SEQ
Allele Specific Protocols
Sanger sequencing
健康报告 屏障设施级别描述 >
 
繁育策略
同胞兄弟姐妹交配
销售和使用条款

查阅销售和使用条款Info > 

 

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000671
DBA2
近交系
心血管生物学、神经生物学和感觉神经研究
参考文献|
x
  •  
    精选参考文献
    在文章发表时，如使用了该品系，请引用原始文献，并在材料与方法部分注明JAX品系号000671。
    2017
    A Spontaneous Mutation in Taar1 Impacts Methamphetamine-Related Traits Exclusively in DBA/2 Mice from a Single Vendor.
    Reed C , et al.
    PubMed:29403379
    MGI:J:265410
    Pituitary 4(1-2):79-86
    2017
    Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains.
    Timmermans S , et al.
    PubMed:28784771
    MGI:J:244295
    Proc Natl Acad Sci U S A 114(34):9158-9163
    2013
    Deficiency of complement component 5 ameliorates glaucoma in DBA/2J mice.
    Howell GR , et al.
    PubMed:23806181
    MGI:J:199369
    J Neuroinflammation 10(1):76
    2013
    Retinal ganglion cell dendritic atrophy in DBA/2J glaucoma.
    Williams PA , et al.
    PubMed:23977271
    MGI:J:199858
    PLoS One 8(8):e72282
    2008
    Mouse genetic models: an ideal system for understanding glaucomatous neurodegeneration and neuroprotection.
    Howell GR , et al.
    PubMed:18929118
    MGI:J:140472
    Prog Brain Res 173:303-21
    2004
    Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse.
    Petkov PM , et al.
    PubMed:15081119
    MGI:J:89298
    Genomics 83(5):902-11
    2002
    Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice.
    Anderson MG , et al.
    PubMed:11743578
    MGI:J:75398
    Nat Genet 30(1):81-5
    2001
    Genetic loci determining bone density in mice with diet-induced atherosclerosis.
    Drake TA , et al.
    PubMed:11328966
    MGI:J:69682
    Physiol Genomics 5(4):205-15
    1995
    Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes.
    Kirk EA , et al.
    PubMed:7595076
    MGI:J:28648
    J Lipid Res 36(7):1522-32
    1995
    Genetics of responsiveness to high-fat and high- cholesterol diets in the mouse.
    Paigen B , et al.
    PubMed:7625360
    MGI:J:28248
    Am J Clin Nutr 62(2):458S-462S
    1993
    On the relationship between D2 receptor density and neuroleptic-induced catalepsy among eight inbred strains of mice.
    Kanes SJ , et al.
    PubMed:7901398
    MGI:J:16201
    J Pharmacol Exp Ther 267(1):538-47
    1993
    Atherosclerosis and plasma and liver lipids in nine inbred strains of mice.
    Nishina PM , et al.
    PubMed:8355588
    MGI:J:13267
    Lipids 28(7):599-605
    1990
    Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice.
    Paigen B , et al.
    PubMed:2317166
    MGI:J:22615
    Arteriosclerosis 10(2):316-23
    1985
    Variation in susceptibility to atherosclerosis among inbred strains of mice.
    Paigen B , et al.
    PubMed:3841001
    MGI:J:109950
    Atherosclerosis 57(1):65-73
  •  
    其它参考文献
    2011
    Mouse genomic variation and its effect on phenotypes and gene regulation.
    Keane TM , et al.
    PubMed:21921910
    MGI:J:177037
    Nature 477(7364):289-94
    2007
    Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains.
    Moy SS , et al.
    PubMed:16971002
    MGI:J:138682
    Behav Brain Res 176(1):4-20
    2004
    Strain distribution pattern of susceptibility to immune-mediated nephritis.
    Xie C , et al.
    PubMed:15067087
    MGI:J:122988
    J Immunol 172(8):5047-55
    2003
    Allergen-induced airway disease is mouse strain dependent.
    Whitehead GS , et al.
    PubMed:12626335
    MGI:J:84265
    Am J Physiol Lung Cell Mol Physiol 285(1):L32-42
    2000
    Differential maintenance and frequency-dependent tuning of LTP at hippocampal synapses of specific strains of inbred mice.
    Nguyen PV , et al.
    PubMed:11067991
    MGI:J:71278
    J Neurophysiol 84(5):2484-93
    2000
    Macronutrient diet selection in thirteen mouse strains.
    Smith BK , et al.
    PubMed:10749765
    MGI:J:61602
    Am J Physiol Regul Integr Comp Physiol 278(4):R797-805
    1999
    Interacting loci cause severe iris atrophy and glaucoma in DBA/2J mice.
    Chang B , et al.
    PubMed:10192392
    MGI:J:54013
    Nat Genet 21(4):405-9
    1998
    Essential iris atrophy, pigment dispersion, and glaucoma in DBA/2J mice [published erratum appears in Invest Ophthalmol Vis Sci 1998 Aug;39(9):1641]
    John SW , et al.
    PubMed:9579474
    MGI:J:47302
    Invest Ophthalmol Vis Sci 39(6):951-62
    1998
    Exposure to an augmented acoustic environment alters auditory function in hearing-impaired DBA/2J mice.
    Turner JG , et al.
    PubMed:9606065
    MGI:J:47560
    Hear Res 118(1-2):101-13
    1996
    Morphological changes in the anteroventral cochlear nucleus that accompany sensorineural hearing loss in DBA/2J and C57BL/6J mice.
    Willott JF , et al.
    PubMed:8852372
    MGI:J:32170
    Brain Res Dev Brain Res 91(2):218-26
    1993
    Voluntary consumption of morphine in 15 inbred mouse strains.
    Belknap JK , et al.
    PubMed:7871041
    MGI:J:15468
    Psychopharmacology (Berl) 112(2-3):352-8
    1993
    Genetics of age-related hearing loss in mice: I. Inbred and F1 hybrid strains.
    Erway LC , et al.
    PubMed:8458745
    MGI:J:11837
    Hear Res 65(1-2):125-32
    1992
    Dietary obesity in nine inbred mouse strains.
    West DB , et al.
    PubMed:1621856
    MGI:J:1348
    Am J Physiol 262(6 Pt 2):R1025-32
    1990
    Deficiency of the murine fifth complement component (C5). A 2-base pair gene deletion in a 5'-exon.
    Wetsel RA , et al.
    PubMed:2303408
    MGI:J:23983
    J Biol Chem 265(5):2435-40
    1989
    Independence of uniphasic and biphasic audiogenic seizure progressions in mice.
    Reid HM , et al.
    PubMed:2590151
    MGI:J:27065
    Behav Neural Biol 52(3):417-21
    1987
    The fifth component of complement (C5) in the mouse. Analysis of the molecular basis for deficiency.
    Wheat WH , et al.
    PubMed:3572304
    MGI:J:8690
    J Exp Med 165(5):1442-7
    1984
    Spontaneous calcified tongue lesions in DBA mice.
    Matsushima Y , et al.
    PubMed:6519193
    MGI:J:166811
    Jikken Dobutsu 33(4):539-42
    1983
    Genetics of beta-2 microglobulin in the mouse.
    Michaelson J , et al.
    PubMed:6187676
    MGI:J:7014
    Immunogenetics 17(3):219-60
    1979
    Genetic defect in secretion of complement C5 in mice.
    Ooi YM , et al.
    PubMed:492335
    MGI:J:6214
    Nature 282(5735):207-8
    1975
    Heritability of the phytohemagglutinin responsiveness of lymphocytes and its relationship to leukemogenesis.
    Heiniger HJ , et al.
    PubMed:163691
    MGI:J:22608
    Cancer Res 35(3):825-31
    1973
    Genetic and biochemical studies of the adrenal lipid depletion phenotype in mice.
    Doering CH , et al.
    PubMed:4348256
    MGI:J:5333
    Biochem Genet 8(1):101-11
    1972
    Genetic relationships between inbred strains of mice.
    Taylor BA , et al.
    PubMed:5031317
    MGI:J:24288
    J Hered 63(2):83-6
    1968
    Reticulum cell neoplasm, type B, or the Hodgkin's-like lesion of the mouse.
    Dunn TB , et al.
    PubMed:4869134
    MGI:J:2417
    J Natl Cancer Inst 40(4):771-821
    1967
    Audiogenic seizures in eleven mouse strains.
    Fuller JL , et al.
    PubMed:6055327
    MGI:J:24264
    J Hered 58(3):135-40
    1967
    Deficiency of the fifth component of complement in mice with an inherited complement defect.
    Nilsson UR , et al.
    PubMed:4959665
    MGI:J:5016
    J Exp Med 125(1):1-16
    1952
    COMMITTEE on Standardized Nomenclature for Inbred Strains of Mice
    International Nomenclature Committee , et al.
    PubMed:14945054
    MGI:J:166288
    Cancer Res 12(8):602-13
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    等位基因参考文献
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